Description
The ExcelDye™ 6× DNA Loading Dye (Orange) is pre-mixed buffer for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains one dye (Orange G) for tracking the DNA migration. The Orange G migrates at approximately 30 bp on a standard 2% TAE agarose gel (50 bp on 1% TAE agarose gel). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.
Composition
0.15% Orange G
10 mM Tris-HCl (pH 8.0)
60% glycerol
60 mM EDTA
Storage
4°C for 12 months
-20°C for 36 months
Contents
Component |
Volume |
ExcelDye 6X DNA Loading Dye, Orange |
5ml x 2 |
Atsushi Yamaguchi 1, Keisuke Takanashi 1 Sci Rep. 2016; 6: 35195. Published online 2016 Oct 12. doi: 10.1038/srep35195
PMCID: PMC5059712
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Sensitivity up to 0.14 ng DNA or 1 ng total RNA
A safer alternative to EtBr
Suitable to blue or UV light
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Sensitivity up to 0.04 ng DNA
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Safety dye
Convenience - monitor the electrophoresis in real-time