[TQ1101] ExcelTaq™ 2倍高特異性染劑定量PCR檢測試劑盒(無參比染料) , 500 RXN

Description

The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and template. The master mix features high sensitivity and signal intensity as well as low background and better compatibility with cDNA templates derived directly from reverse transcription reaction mixture. The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) contains hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. This master mix allows for sensitive, precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules. With inert smart blue contrast dye, the ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process. The ExcelTaq™ 2X Q-PCR Master Mix is also compatible with a ROX reference dye if recommended by the manufacturer of the qPCR system.

Features

High sensitivity and signal intensity
Smart blue contrast dye as a visual aid for reaction setup
Better compatibility for reverse transcription
Low background

Storage

Protected from light.
Aliquot to avoid multiple freeze-thaw cycles.
-20°C for 12 months

Sensitivity

The amplification plot of real-time PCR with cDNA template ranged from 25 ng to 1.49 fg in quantity, analyzed by using TQ1100 ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) for Q-PCR amplification.

Better Compatibility for reverse transcription

ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) shows better compatibility with reverse-transcription reaction mixture as compared to similar products from A and B brands. Two μl of cDNA directly obtained from reverse-transcription reaction mixture were used in a 20 μl qPCR reaction for the compatibility test.

Specificity

The standard curve (left) and melting curve (right) of TQ1100 ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX).

TaqMan® vs. SYBR® Green Chemistry

How can I choose ExcelTaq™ 2X Q-PCR Master Mix?

What instruments are compatible with ExcelTaq™ 2X Q-PCR Master Mix ?

Product Name

ExcelTaq™ 2X Q-PCR Master Mix
(SYBR, no ROX)

ExcelTaq™ 2X Q-PCR Master Mix
(SYBR, ROX)

ExcelTaq™ 2X Fast Q-PCR Master Mix
(SYBR, no ROX)

ExcelTaq™ 2X Fast Q-PCR Master Mix
(SYBR, ROX)

ExcelTaq™ 2X Q-PCR Master Mix
(TaqMan, ROX)

Cat. No.

TQ1100

TQ1110

TQ1200

TQ1210

TQ2110

Detection chemistry

SYBR

TaqMan

Blue contrast dye for best convenience

P

X

ROX dye for reference

X

P

X

P

Time required for enzyme activation

10 min

2 min

10 min

qPCR program

Standard

Fast and Standard

Standard

Instrument Compatibility*

Thermo Fisher (including Applied Biosystems system and Life Technologies):

5700, 7000, 7300, StepOne™, StepOne™ plus

Δ

V

Δ

V

7500, 7500 FAST, QuantStudio™ 1, 3, 5, 6, 7, 12k Flex, ViiA7™

Δ

7700, 7900, 7900HT,

7900HT FAST

Δ

V

Δ

V

Piko Real®

V

BioRad system:

CFX96™, CFX384™,

CFX Connect™

V

Chromo4™, MiniOpticon™, Opticon™, Opticon™ 2

V

iCycler®, iQ™ 5, MyiQ™

V

Qiagen (Corbett):

Rotor-Gene™ 3000,

Rotor-Gene™ 6000,

Rotor-Gene™ Q

V

Roche system:

LightCycler® 480,

LightCycler® 96,

LightCycler® Nano

V

Agilent (Stratagene):

AriaMX, AriaDX

Δ

MX3000P®,

MX3005P®,

MX4000P®

Δ

BSM

Mic

V

BJS

Xxpress®

V

Cepheid

SmartCycler®

V

Eppendorf

Mastercycler® ep

realplex 2S

V

Fluidigm

BioMark™

Δ

Hain Lifescience

FluoroCycler® 96

V

IT-IS Life Science

MyGo Pro,

MyGo Mini

V

PCRmax

Eco™

V

Takara

Thermal Cycler

Dice®

V

TP8000

V

Techne

PrimeQ, Quantica®

V

Analytik Jena

qTOWER³

V

Bioevopeak

Real-time PCR system,

PCR-Q96 Series

V

EiNS Sci

E-QPCR4-CI,

E-QPCR6-CI

V

*ExcelTaq™ 2X Q-PCR Master Mix can be used with a variety of real-time instruments, including but not limited to the list above.

V– Indicates the reagent is compatible to the instrument

Δ– Indicates that if use the reagent to the instrument, ensure to de-select passive dye channel when data processing.

Can you provide the results that using ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX, TQ1100) with different instruments?

Lactate modulates microglia polarization via IGFBP6 expression and remodels tumor microenvironment in glioblastoma

Lucia Longhitano, Nunzio Vicario, Stefano Forte, Cesarina Giallongo, Giuseppe Broggi, Rosario Caltabiano, Giuseppe Maria Vincenzo Barbagallo, Roberto Altieri, Giuseppina Raciti, Michelino Di Rosa, Massimo Caruso, Rosalba Parenti, Arcangelo Liso, Federica Busi, Marco Lolicato, Maria Caterina Mione, Giovanni Li Volti, Daniele Tibullo, Cancer Immunol Immunother. 2022 Jun 3. doi: 10.1007/s00262-022-03215-3. Online ahead of print.

PMID: 35654889

Prevention, inhibition, and degradation effects of melittin alone and in combination with vancomycin and rifampin against strong biofilm producer strains of methicillin-resistant Staphylococcus epidermidis

Rasoul Mirzaei, Mohammad Yousef Alikhani, Carla Renata Arciola, Iraj Sedighi, Rasoul Yousefimashouf, Kamran Pooshang Bagheri, Biomed Pharmacother. 2022 Mar;147:112670. doi: 10.1016/j.biopha.2022.112670. Epub 2022 Feb 3.

PMID: 35123230

Gut Microbiota Modulation of Moderate Undernutrition in Infants through Gummy Lactobacillus plantarum Dad-13 Consumption: A Randomized Double-Blind Controlled Trial

Rafli Zulfa Kamil, Agnes Murdiati, Mohammad Juffrie, Endang Sutriswati Rahayu, Nutrients. 2022 Mar 1;14(5):1049. doi: 10.3390/nu14051049.

PMCID: PMC8912314

Integrative Metabolomic and Lipidomic Profiling of Lung Squamous Cell Carcinoma for Characterization of Metabolites and Intact Lipid Species Related to the Metastatic Potential

Hamid Reza Rafieian-Naeini, Mahdi Zhandi, Mostafa Sadeghi, Ali Reza Yousefi, Andrew Parks Benson, Poult Sci. 2021 Nov;100(11):101418. doi: 10.1016/j.psj.2021.101418. Epub 2021 Aug 8.

PMCID: PMC8531857

Effects of coenzyme Q10 on reproductive performance of laying Japanese quail (Coturnix japonica) under cadmium challenge

Heayyean Lee, Hwanhui Lee, Sujeong Park, Myeongsun Kim, Ji Young Park, Hanyong Jin, Kyungsoo Oh, Jeehyeon Bae, Young Yang, Hyung-Kyoon Choi, Cancers (Basel). 2021 Aug 19;13(16):4179. doi: 10.3390/cancers13164179.

PMCID: PMC8391613

Using de novo transcriptome assembly and analysis to study RNAi in Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae)

Satnam Singh,corresponding author Mridula Gupta, Suneet Pandher, Gurmeet Kaur, Neha Goel, and Pankaj Rathore

Sci Rep. 2019; 9: 13710. Published online 2019 Sep 23. doi: 10.1038/s41598-019-49997-y

PMCID: PMC6757040

Reference Gene Selection in Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) and Their Normalization Impact on Gene Expression in RNAi Studies.

Satnam Singh Suneet Pandher Mridula Gupta Gurmeet Kaur Pankaj Rathore

Journal of Economic Entomology, toy328, Published: 17 October 2018 doi: 10.1093/jee/toy328

Synergistic inhibition effect of TNIK inhibitor KY-05009 and receptor tyrosine kinase inhibitor dovitinib on IL-6-induced proliferation and Wnt signaling pathway in human multiple myeloma cells

Yura Lee, Jung-Il Jung, Kyeong-Yong Park, Soon Ae Kim, Jiyeon Kim Oncotarget. 2017 Jun 20; 8(25): 41091–41101. Published online 2017 Apr 12. doi: 10.18632/oncotarget.17056

PMCID: PMC5522218

Hydroxylation and sulfation of sex steroid hormones in inflammatory liver

Sang R. Lee, Seung-yeon Lee, Sang-yun Kim, Si-yun Ryu, Bae-kuen Park, Eui-Ju Hong J Biomed Res. 2017; 31(5): 437–444. doi: 10.7555/JBR.31.20170031

PMCID: PMC5706436

ExcelTaq™ 2X Q-PCR Master Mix

Product Name	ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX)	ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX)	ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX)	ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX)	ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX)
Cat. No.	TQ1100	TQ1110	TQ1200	TQ1210	TQ2110
Detection chemistry	SYBR	SYBR	SYBR	SYBR	TaqMan
Blue contrast dye for best convenience	ü	ü	ü	ü	X
ROX dye for reference	X	ü	X	ü	ü
Time required for enzyme activation	10 min	10 min	2 min	2 min	10 min
qPCR program	Standard	Standard	Fast and Standard	Fast and Standard	Standard